Gilson Connect
Solid phase reversible immobilization (SPRI) beads are commonly used to clean up polymerase chain reaction (PCR) and DNA samples for downstream applications, such as sequencing and cloning. This application note demonstrates a flexible and reliable automated procedure for next-generation sequencing (NGS) library cleanup.
Physicians need test kits to identify all of the genetic mutations of a condition at once so that they can initiate the right treatment. In this app note, we describe how the PIPETMAX automated liquid handler can be used within a BRCA MASTR Dx molecular diagnostic assay.
Reagents used in qPCR master mixes, including buffers, surfactants, and detergents, can negatively influence accuracy and precision, thereby increasing sample variability and decreasing data reliability. In this app note, we used PIPETMAX® to test whether the number of times a tip was pre-wet before transferring master mix affected qPCR performance.
The Bradford Assay is a common universal protein concentration determination technique. In this app note, you'll learn how the automation of this qualitative application shows comparability between manual and automated results for preparing a standard curve and samples for quantification of protein in solution.
In this application, NGS libraries were prepared from E. Coli DH10B genomic DNA (gDNA) and FAMlabeled adaptors using the NxSeq® DNA Sample Prep kit, comparing the manual method with an automated library prep protocol on the PIPETMAX. This application note describes the results of the ligation efficiency of manual library preparation versus automated library preparation.
In this application note we examine the limits of quantification and detection, repeatability, reproducibility, and recovery in food safety analytical testing. Automation with the GX-271 ASPEC® system provides a reproducible and
reliable method for the isolation.
In this application note, three glucocorticoids and three nonsteroidal anti-inflammatory drugs were detected simultaneously from horse urine using the Gilson GX-274 ASPEC® system with two step extractions and LC/MS/MS selected reaction monitoring (SRM).
Deoxynivalenol (DON) is a mycotoxin that occurs predominantly in grains such as wheat, barley, oats, rye, and maize and consumption is associated with outbreaks of gastroenteritis in humans. Automated methods are well-suited for quantification of toxins because they are accurate, reduce the time required for laboratory analysis, and reduce human errors - learn more in this application note.
Nitrosamines are a class of semi-volatile organic chemical compounds characterized by a nitroso group attached to an amine group. In this tech note we describe a method for automating US EPA Method 521 using the Gilson ASPEC® 274 Large Volume System
There are many considerations when moving from manual to automated mAb purification, and this technical note discusses some of the hardware and software considerations for maintaining sample integrity and reducing carryover when using a Gilson automated liquid handling system.
